Eurofins Genomics

 Whole plasmid sequencing (WPS) is becoming a gold-standard method for validating plasmid constructs with high accuracy and complete coverage. Unlike traditional Sanger sequencing, which only reads short fragments, whole plasmid sequencing provides a full, error-free view of the entire plasmid. This makes it essential for molecular biology, gene therapy, synthetic biology, and biotech research where plasmid integrity is critical. In this blog, we explore what whole plasmid sequencing is, how it works, why it matters, and when researchers should choose it over conventional sequencing methods. What Is Whole Plasmid Sequencing? Whole plasmid sequencing is a next-generation sequencing (NGS) method used to read the entire plasmid sequence in a single workflow. This includes: Promoters Antibiotic resistance genes Inserts and payload sequences Vector backbone Cloning sites Origins of replication Unlike Sanger sequencing, which may leave gaps, whole plasmid sequen...